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  • FAQ: How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?

    Both OneTaq and LongAmp Taq DNA polymerases are blends of Taq and Deep Vent DNA Polymerases. Both enzyme blends take advantage of the observation that the addition of a proofreading (exo+) polymerase to Taq increases performance [Barnes, W.M. (1994) Proc. Natl. Acad. Sci. USA, 91, 2216-2220], however the concentration and ratio of the enzymes is different in each blend. For LongAmp Taq, the supplied buffer, enzyme concentrations and ratio have been adjusted to favor robust amplification of moderate to very long products (≤ 30 kb lambda or human genomic). OneTaq and the OneTaq, along with the OneTaq Standard Buffer, GC Buffer and High GC Enhancer, have all been formulated for robust performance of short to moderate length products (≤ 6kb genomic) across very low to very high GC content (~15-85%).