FAQ: What thermostable DNA polymerase can be used for PCR after cDNA synthesis?

For downstream PCR, we recommend OneTaq 2X Master Mix (NEB #M0482 or M0485) for PCR detection up to 5kb, Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494) for highest fidelity, or LongAmp Taq 2X Master Mix (NEB #M0287) for high yields from longer products.