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  • FAQ: What are the advantages to using Q5® Hot Start High-Fidelity DNA Polymerase?

    Q5 Hot Start High-Fidelity DNA Polymerase's processivity-enhancing domain and robust buffer system provides high-fidelity amplification of a wide variety of targets: from high AT to high GC, from small amplicons to up to 10kb human genomic and at least 20kb simple genomic DNA. The hot start formulation inhibits the robust exonuclease activity of the enzyme, allowing for convenient room temperature reaction set up. Q5 Hot Start is suitable for all PCR applications requiring greater accuracy, high specificity, or the amplification of difficult or long targets.