Q5 High-Fidelity 2X Master Mix combines high-fidelity amplification from a wide variety of targets (from high AT to high GC amplicons, from small to up to 10kb human genomic amplicons) with the convenience of a master mix formulation. Q5 is suitable for all PCR applications requiring greater accuracy, or the amplification of difficult or long targets. The master mix contains enzyme, Mg²⁺, dNTPs, and all necessary buffer components to support robust amplification - only template and primers need to be added.