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  • FAQ: Is NEBNext® High-Fidelity 2X PCR Master Mix the same as the Q5® High-Fidelity Master Mix?

    No. Although both master mixes contain Q5 High-Fidelity DNA Polymerase, the master mix composition is different. NEBNext High-Fidelity 2X PCR Master Mix has been specifically optimized for amplification of NGS libraries, while Q5 High-Fidelity Master Mix has been optimized for amplification of a variety of templates.