FAQ: I have a tube of the Q5® High GC Enhancer from another product formulation - can I add it to the Q5 Master Mix?

No, we do not recommend adding the Enhancer to the Master Mix. The Q5 2X Master Mixes have been specially formulated for robust performance on templates that range from high AT to high GC. Addition of the Enhancer is more likely to reduce performance of the mixes than improve it.