Home FAQs How should I set up a PCR reaction using Q5® Hot Start High-Fidelity DNA Polymerase?

FAQ: How should I set up a PCR reaction using Q5® Hot Start High-Fidelity DNA Polymerase?

The general guidelines for a 50 µl reaction are:
1 unit Q5 Hot Start High-Fidelity DNA Polymerase
200 μM each dNTP
0.5 μM each primer
2-50 pg plasmid or 50-500 ng genomic template
1X Q5 Reaction Buffer
(1X High GC Enhancer - optional)
Denature at 98°C
Extend 20 s/kb at 72°C 

Reactions can be set up at room temperature