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  • FAQ: How should I set up a PCR reaction using Q5® Hot Start High-Fidelity DNA Polymerase?

    The general guidelines for a 50 µl reaction are:
    1 unit Q5 Hot Start High-Fidelity DNA Polymerase
    200 μM each dNTP
    0.5 μM each primer
    2-50 pg plasmid or 50-500 ng genomic template
    1X Q5 Reaction Buffer
    (1X High GC Enhancer - optional)
    Denature at 98°C
    Extend 20 s/kb at 72°C 

    Reactions can be set up at room temperature