FAQ: How should I set up a PCR experiment using Q5® High-Fidelity 2X Master Mix?

The general guidelines for a 50 µl reaction are:
0.5 μM each primer
2-50 pg plasmid or 50-500 ng genomic template
1X Q5 High-Fidelity 2X Master Mix
Denature at 98°C
Extend 20 s/kb at 72°C 

Reactions should be set up on ice