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  • FAQ: How should I set up a PCR experiment using Q5® High-Fidelity 2X Master Mix?

    The general guidelines for a 50 µl reaction are:
    0.5 μM each primer
    2-50 pg plasmid or 50-500 ng genomic template
    1X Q5 High-Fidelity 2X Master Mix
    Denature at 98°C
    Extend 20 s/kb at 72°C 

    Reactions should be set up on ice