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  • FAQ: Can I use the NEBNext DNA Library Prep Reagent Set for Illumina reagents for preparing libraries for sequencing on the Roche 454 GSFLX?

    No. The adapters provided by Roche have blunt ends rather than 5'-T overhangs. In addition, not all the reagents required for the Roche 454 GSFLX sample preparation are included in this reagent set.