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  • FAQ: When should Bst 2.0 DNA Polymerase be the enzyme of choice?

    Bst 2.0 DNA Polymerase displays high strand displacement activity. It fills a void between thermophilic and mesophilic polymerases. The temperature optimum of 60-70°C is higher than DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210) and lower than Vent DNA Polymerase (NEB# M0254), two other strand displacing polymerases. This gives researchers a wider range of reaction conditions to optimize strand displacement and primer annealing. This is useful in the design of sequencing strategies as well as isothermal amplification technologies. The elevated reaction temperature facilitates sequencing through GC rich regions.