HomeFAQsWhat are the main causes of reaction failure using Bst 2.0 DNA Polymerase?
FAQ: What are the main causes of reaction failure using Bst 2.0 DNA Polymerase?
Temperatures over 72 °C can significantly reduce enzyme activity. In addition, failure to use the appropriate buffer containing either 0.1% Triton X -100, 0.1% Tween-20 or 100 μg/ml BSA can cause reaction failure. See Q3 above.