• My NEB
  • Print
  • PDF
  • FAQ: Can T3 DNA Ligase be heat inactivated?

    Yes, heating a reaction containing T3 DNA Ligase at 65 °C for 10 minutes will inactivate the enzyme. However, the reaction needs to be performed in a buffer without PEG. Do not heat inactivate if there is PEG in the reaction buffer, as transformation will be inhibited.