HomeFAQsCan Bst DNA 2.0 Polymerase be used in other NEBuffers?
FAQ: Can Bst DNA 2.0 Polymerase be used in other NEBuffers?
Optimal activity is observed in Isothermal Amplification Buffer and near optimal performance is observed in ThermoPol™ reaction buffer. The unit is assayed in a non-optimal buffer taken from the literature. The enzyme exhibits 25% activity in NEBuffer 1 and 100% activity in buffers 2, 3, 4, and EcoRI. NEBuffers 1-4 should be supplemented with 0.1% Triton X-100, 0.1% Tween-20 or 100 µg/ml BSA to achieve best results.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.