FAQ: Can Bst 2.0 DNA Polymerase be used at temperatures other than 65°C?

Yes, the enzyme has the following activities:
10-15% at 37°C
30-50% at 50°C
100% at 60-70°C
30-50% at 72°C
Use of Bst 2.0 is not recommended at temperatures greater than 72°C because it becomes heat-inactivated.