HomeFAQsDo I need to transform the Instant Sticky-end Ligase Master Mix reactions immediately?
FAQ: Do I need to transform the Instant Sticky-end Ligase Master Mix reactions immediately?
No. The ligation/transformation efficiency does not decrease if the ligation reactions are kept at room temperature or at 4°C for a few minutes. We do not recommend incubations longer than 1 hour. If the DNA will not be used for transformation within 1 hour, we recommend storing it at -20°C.