New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsCan the Instant Sticky-end Ligase Master Mix be used for the ligation of blunt-end or single-base overhang fragments?
FAQ: Can the Instant Sticky-end Ligase Master Mix be used for the ligation of blunt-end or single-base overhang fragments?
Yes, but the reaction will no longer be instantaneous. An incubation time of 15-30 minutes at room temperature is required to ensure high efficiency ligation of blunt ends or single-base overhangs.
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