FAQ: Will Q5® High-Fidelity DNA Polymerase incorporate dUTPs?

No. Q5 High-Fidelity DNA Polymerase will not incorporate dUTPs and is not compatible with USER™ cloning methods or bisulfite-treated DNA. For protocols employing bisulfite-treated DNA, we recommend EpiMark® Hot Start DNA Polymerase (NEB# M0490).