FAQ: What is the fidelity of Q5® High-Fidelity DNA Polymerase?

Q5 High-Fidelity DNA Polymerase has a fidelity >100X Taq DNA Polymerase.

Due to the very low frequency of misincorporation events being measured, the error rate of high-fidelity enzymes like Q5 is difficult to measure in a statistically significant manner. Although measurements from assays done side-by-side with Taq yield a Q5 fidelity value of approximately 200X Taq, we report ">100X Taq" as a conservative value.