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  • FAQ: My template is GC rich or supercoiled. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?

    The Q5 High GC Enhancer can improve yields of high GC or difficult amplicons. The Enhancer is NOT a buffer and should be added to the Q5 reaction mix (containing Q5 Reaction Buffer) at a final 1X concentration. The Q5 High GC Enhancer typically benefits high GC amplicons but can be inhibitory to high AT amplicons.