HomeFAQsHow should I set up a PCR experiment using Q5® High-Fidelity DNA Polymerase?
FAQ: How should I set up a PCR experiment using Q5® High-Fidelity DNA Polymerase?
The general guidelines for a 50 µl reaction are:
1 unit Q5 High-Fidelity DNA Polymerase
200 μM each dNTP
0.5 μM each primer
2-50 pg plasmid or 50-500 ng genomic template
1X Q5 Reaction Buffer
(1X High GC Enhancer - optional)
Denature at 98°C
Extend 20 s/kb at 72°C
Reactions should be set up on ice
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.