FAQ: What type of sample prep/library construction can I use NEBNext DNA Library Prep Master Mix Set for Illumina for?

The NEBNext DNA Library Prep Master Mix Set for Illumina (I) is compatible with protocols requiring a 3´ A overhang on the library molecules to facilitate ligation to adapters containing a 5´ T overhang, such as Illumina’s Genomic DNA Sample Prep protocol for the Genome Analyzer II.