• My NEB
  • Print
  • PDF
  • FAQ: Do you have some references on IPL?

    The following references on IPL may be useful:

    Reviews

    Ghosh, I., Considine, N., Maunus, E., Sun, L. , Zhang, A., Buswell, J. , Evans, T.C. Jr. and Xu, M.Q. (2011). Site-specific protein labeling by intein-mediated protein ligation. Methods Mol Biol. 705:87-107.

    Berrade, L.; Camarero, J. A. (2009) Expressed protein ligation: a resourceful tool to study protein structure and function. Cell Mol Life Sci 66(24): 3909-22.

    Xu, M.-Q., and Evans, T. C. Jr. Intein-mediated ligation and cyclization of Expressed Proteins. Methods 24, 257-277. 

    Xu, M.Q., Paulus, H. and Chong, S. (2000) Fusions to self-splicing inteins for protein purification. Methods Enzymol. 326:376-418. 

    Evans T. C. & Xu, M.-Q., (1999) Intein-mediated protein ligation: harnessing nature's escape artists. Biopolymers 51, 333-42. 

    pTXB1,3

    Evans, T.C. Jr., Benner, J., and Xu, M.-Q. (1998) Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci. 7, 2256-2264.

    pTWIN1,2

    Evans, T.C. Jr., Benner, J., and Xu, M.-Q.(1999) The cyclization and polymerization of bacterially-expressed proteins using modified self-splicing inteins. J. Biol. Chem. 274, 18359-18363.

    Evans, T.C. Jr., Benner, J., and Xu, M.-Q. (1999) The in vitro ligation of bacterially expressed protein using an intein from Methanobacterium thermoautotrophicum. J. Biol. Chem. 274, 3923-3926.

    Mathys, S., Evans, T.C. Jr., Chute, I.C., Wu, H., Chong, S., Benner, J., Liu, X.-Q., Xu, M.-Q. (1999) Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene, 231:1-13.

    Applications

    Kochinyan, S.; Sun, L.; Ghosh, I.; Barshevsky, T.; Xu, J.; Xu, M. Q. (2007) Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phosphatases. Biotechniques 42(1): 63-9.

    Sun, L., Rush, J., Ghosh, I., Maunus, J.R. and Xu, M.-Q. (2004) Producing peptide arrays for epitope mapping by intein-mediated protein ligation. Biotechniques. 37:430-443.

    Ghosh, I, Sun, L., Evans, T.C. Jr., and Xu, M.-Q. (2004) An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays. J. of Imm. Methods. 293:85-95.

    Xu, J., Sun, L., Ghosh, I., and Xu, M.-Q. (2004) Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein. Biotechniques. 36:976 -981 .

    For an intein bibliography please refer to Inbase, the Intein database