FAQ: Can I use the NEBNext DNA Library Prep Master Mix Set for Illumina reagents for preparing libraries for sequencing on the Roche 454 GS FLX ?

No. The adapters provided by Roche have blunt ends rather than 5´-T overhangs. In addition, not all the reagents required for the Roche 454 GSFLX sample preparation are included in this reagent set.