FAQ: EciI used to be supplied with NEBuffer 2, but now the activity chart indicates that the enzyme only has 50% activity in this buffer. Why?

NEB now sells a recombinant form of EciI. This recombinant protein has 50% activity in NEBuffer 2 if BSA is not added to the reaction. If BSA is added, recombinant EciI has 100% activity in NEBuffer 2.

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