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  • FAQ: Is OneTaq® Hot Start DNA Polymerase active in other Taq reaction buffers?

    The OneTaq® Standard and GC Reaction Buffers have been optimized for robust performance over a wide range of target amplicons. For routine/easy targets, other Taq Reaction Buffers can be used successfully, however for high AT or GC amplicons they typically result in decreased reaction specificity and/or suboptimal yields.