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  • FAQ: What is the composition of the Standard Taq Reaction Buffer?

    1X Standard Taq Reaction Buffer: 10 mM Tris-HCl, 50 mM KCl, and 1.5 mM MgCl2, pH 8.3 @ 25°C. Supplied as a 10X concentrated stock. For reactions requiring dNTPs, Deoxynucleotide Solution Mix (NEB #N0447) or Deoxynucleotide Solution Set (NEB #N0446) are available separately.