HomeFAQsWhat is the best way to generate partials using T7 Exonuclease?
FAQ: What is the best way to generate partials using T7 Exonuclease?
The enzyme should be titered to the substrate. Serial dilution of the DNA incubated for a specific time is suggested. Start with a 30 minute incubation. Incubating at room temperature can be used to slow the reaction. If the DNA is limiting the enzyme can be diluted in reaction buffer for immediate use.