FAQ: Is there variability in the cleavage site for MnlI?

There appears to be a wobble effect that imitates star activity, and represents a variable cleavage site. MnlI always leave a single-base 3´ overhang. The majority of sites under our unit definition conditions will cut (7/6) from the recognition site, but some sites have been reported to cut at (7/8) from the recognition site.

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MnlI