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  • FAQ: What is the half-life of the Gaussia luciferase (GLuc)?

    The half-life of GLuc remains unclear. All GLuc-expressing vectors available for mammalian expression at NEB harbor the humanized codons for GLuc in which the secreted GLuc has activity similar to that of the native protein. Once GLuc is secreted into the culture medium, it is very stable. For example, ~20% of the GLuc activity was detectable after incubating a GLuc-containing sample at 99˚C for 15 minutes.