HomeFAQsWhy can a cleaved MmeI site religate but not be re-cut?
FAQ: Why can a cleaved MmeI site religate but not be re-cut?
MmeI is an unusual enzyme in that it has both restriction and methylation properties. Because DNA cleavage does not break the recognition site, the enzyme can methylate its recognition site following cleavage. Once the site is methylated, it can not be subsequently cut after being ligated.