HomeFAQsWhat are some of the possible explanations for an inability to clone an insert into a pMAL vector?
FAQ: What are some of the possible explanations for an inability to clone an insert into a pMAL vector?
The most common explanation for this is technical difficulties with the subcloning. Another explanation is that expression of the fusion is toxic to E. coli. The tac promoter induction ratio on the pMAL plasmids is about 1:30, so if the induced level of the fusion is 40% of the total cellular protein, the uninduced level works out to over 1%. This amount of a protein can be toxic, either because of its function (e.g., a protease) or because of its general properties (e.g., very hydrophobic).
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.