FAQ: Will CIP work in restriction enzyme NEBuffers, including CutSmart?

CIP is active in all restriction enzyme NEBuffers 1.1, 2.1, 3.1 as well as NEBuffers 1, 2, 3, 4, NEBuffer for EcoRI, and CutSmart Buffer. It can be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary. To see its % functional activity in CutSmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.