NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

FAQ: Will CIP work in restriction enzyme NEBuffers, including CutSmart?

CIP is active in all restriction enzyme NEBuffers 1.1, 2.1, 3.1 as well as NEBuffers 1, 2, 3, 4, NEBuffer for EcoRI, and CutSmart Buffer. It can be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary. To see its % functional activity in CutSmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.
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