HomeFAQsI've blunted my sonicated gDNA for 15 minutes instead of the recommended 30 minute incubation time, will the reaction still work?
FAQ: I've blunted my sonicated gDNA for 15 minutes instead of the recommended 30 minute incubation time, will the reaction still work?
A slightly lower transformation efficiency is seen with shorter incubations but the reaction will still work. In general, 1.5 X fewer transformants are seen when PCR product or sheared/nebulized DNA is incubated for 15 minutes instead of 30 minutes. If a 15 minute incubation time is desired, increase blunting mix to 2 µl in the reaction.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.