FAQ: What is MBP5? Is it different from wild-type MBP produced from E. coli?

MBP5 is the protein produced from pMAL-c5X that has a stop codon linker cloned into the XmnI site. It differs from wild-type MBP by the addition of a methionine at the amino terminus (as do all fusions made in pMAL-c vectors), the mutations that increase the affinity of MBP for the amylose resin, the deletion of the last four residues of wild-type MBP, and the addition of the residues encoded by the spacer and the protease site.