HomeFAQsDoes the activity of Cypridina Luciferase interfere with that of other luciferases such as Gaussia, Renilla & Firefly?
FAQ: Does the activity of Cypridina Luciferase interfere with that of other luciferases such as Gaussia, Renilla & Firefly?
No. Cypridina Luciferase catalyzes the light production of a substrate that is different than those used by Gaussia, Renilla or Firefly luciferases. The secreted CLuc is typically assayed in the supernatant of CLuc-expressing cells; however, the CLuc activity can also be assayed in the cell lysate. If the cell lysate contains Gaussia, Renilla or Firefly luciferase in addition to Cypridina Luciferase, one can assay each luciferase independently without cross-reactivity.