HomeFAQsWhat is the difference between the two definitions and why does NEB use the Cohesive End Unit?
FAQ: What is the difference between the two definitions and why does NEB use the Cohesive End Unit?
The Weiss unit is not a direct measurement of DNA ligation; it is an indirect measurement of the enzyme adenylation reaction, followed by determining ATP-PPi exchange. NEB did not think that the Weiss unit was informative enough, because the most prevalent use of T4 DNA ligase is for the ligation of DNA. As the Cohesive End Unit involves the direct measurement of DNA ligation, NEB feels that it more practically relevant.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.