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  • FAQ: What is the difference between EcoRV-HF and EcoRV?

    EcoRV-HF has been engineered to cleave with increased fidelity compared to wild type EcoRV. The Aspartic Acid at position 19 was changed to Alanine and the Glutamic Acid at position 27 was changed to Alanine (D19A/E27A).