FAQ: What is the activity of SssI Methyltransferase in other NEBuffers, including CutSmart?

The activity is 25% in NEBuffer 3 and 10% in NEBuffer 1 and 4. It can also be used in Cutsmart Buffer. To see its % functional activity in Cutsmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.