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  • FAQ: Are there other methods for making probes?

    T7 RNA Polymerase (NEB# M0251) and SP6 RNA Polymerase (NEB# M0207) and the HiScribe™ T7 In Vitro Transcription Kit (NEB# E2030) can be used to make RNA probes. Increased levels of detection in nucleic acid hybridization can be obtained due to the greater stability of RNA: DNA hybrids with respect to DNA: DNA hybrids (1).

    (1) Zinn, K et al. (1983) Cell 34, 865-879.