HomeFAQsIt seems that NdeI is having some difficulties cutting my DNA. Is there a reason for that?
FAQ: It seems that NdeI is having some difficulties cutting my DNA. Is there a reason for that?
NdeI activity is sensitive to contaminants present in DNA isolated with standard miniprep protocols. Further purification of the DNA by a column or dialyzing the contaminant from the DNA may help increase the activity of the enzyme on the isolated DNA substrate.