- Reduce polymerase concentration.
- Shorten extension time.
- Reduce total number of cycles.
- Increase annealing temperature or try 2-step protocol.
- Optimize Mg2+ concentration.
- Lower primer concentration.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.