HomeFAQsCan I assay Gaussia and Firefly luciferase activities if reporter genes are co-transfected in the cells?
FAQ: Can I assay Gaussia and Firefly luciferase activities if reporter genes are co-transfected in the cells?
Yes. Gaussia and Firefly luciferases catalyze the light reaction using different substrates. Therefore, the activity of each luciferase can easily be assayed from the same cells expressing both reporters. The GLuc and the Firefly luciferase activities do not cross-react with each another. The GLuc activity is typically assayed from the supernatant, but it also can be obtained from the cell lysate. The Firefly luciferase activity, on the other hand, can only be assayed from the cell lysate.
Choose your country
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.