FAQ: Will Mung Bean Nuclease degrade double stranded DNA?

When used at temperatures higher than the 30°C recommended, or for longer periods, degradation of the substrate DNA will result. Further, since the endonucleic activity is known to occur at 'bubbles' on the DNA helix, which transiently form along the length of the molecule, substrates which are already nicked, or are of high AT content are more susceptible to this endonucleic activity.