FAQ: Is ClaI an isoschizomer of BspDI?

Yes, ClaI and BspDI recognize the same sequence, cut at the same site, both are blocked by dam methylation, use buffer 4, and are incubated at 37°. ClaI requires 5 units to cleave 1 μg pBR322 DNA versus 1 unit fron BspDI.

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