FAQ: How much Protein Deglycosylation Mix should I use to remove my carbohydrate under native conditions?

When the glycoprotein substrate is not denatured the Protein Deglycosylation Mix may have a more difficult time reaching the cleavage site of the carbohydrate (because of the secondary and tertiary protein structure). Extra enzyme and extended incubation times will help but these values are specific to each protein and must be determined empirically.