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  • FAQ: What are the typical reaction conditions for Endo H?

    Typical reaction conditions are as follows:
    1. Combine 1-20 µg of glycoprotein, 1 µl of 10X Glycoprotein Denaturing Buffer and H2O (if necessary) to make a 10 µl total reaction volume.
    2. Denature glycoprotein by heating reaction at 100°C for 10 minutes.
    3. Make a total reaction volume of 20 µl by adding 2 µl of 10X G5 Reaction Buffer, H2O and 1-5 µl Endo H.
    4. Incubate reaction at 37°C for 1 hour. 

    Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.

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    Products:
    Endo H