1. Combine 1-20 µg of glycoprotein, 1 µl of 10X Glycoprotein Denaturing Buffer and H2O (if necessary) to make a 10 µl total reaction volume.
2. Denature glycoprotein by heating reaction at 100°C for 10 minutes.
3. Make a total reaction volume of 20 µl by adding 2 µl of 10X Glyco Buffer 3, H2O and 1-5 µl Endo H.
4. Incubate reaction at 37°C for 1 hour.
Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.