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FAQ: What buffer should be used with RecAf Protein?

The supplied RecAf buffer can be used to form stable triple helixes when supplemented with ATP gamma-S. ATP gamma-S is not supplied. Sigma product (A1388) ATP gamma-S can be used. A buffer similar to several published in the scientific literature, 25 mM Tris-acetate (pH 7.8), 4 mM Mg+2 acetate, 0.3 mM ATP gamma-S, 0.4 mM DTT, has been successfully used with the RecAf functional assay.

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