• My NEB
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  • FAQ: What are the solutions/recipes (C2984)?

    SOB:
    2% Vegetable peptone (or Tryptone)
    0.5% Yeast Extract
    10 mM NaCl
    2.5 mM KCl
    10 mM MgCl2
    10 mM MgSO4

    SOC:
    SOB + 20 mM Glucose

    LB agar:
    1% Tryptone
    0.5% Yeast Extract
    0.17 M NaCl
    1.5% Agar

    Blue/White Screening:
    X-gal 80 µg/ml
    IPTG* 0.3 mM
    *Omit IPTG for potentially toxic genes