FAQ: Can an abasic site be created and then cleaved with Endonuclease IV in the same reaction? How active is Uracil-DNA-Glycosylase (cat# M0280S) in Endonuclease IV buffer?

The Endonuclease IV buffer is NEBuffer 3. UDG is 100% active in NEBuffer 3 and therefore it is possible to perform both reactions in NEBuffer3.