Yes. McrBC will cut the DNA (both strands) even if the methylated Cs are on only one strand. There must be at least one methylated C in each half-site for McrBC to cut, but the methylated Cs can be on the same or opposite strands. Therefore, if the DNA is methylated on only one strand, it should still be cut by McrBC.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.